Prenatal Ethanol-induced Effect on Cell Count of Pars Distalis of Pituitary Gland in the Rat Pups

Nadia Rashid; M Yunus Khan

Abstract:

Objective: To determine the effects of prenatal administration of ethanol on cell count in pars distalis of pituitary gland in the rat pups. Study design: Experimental study. Place and duration of study: Department of Anatomy, College of Physicians and Surgeons Pakistan, Regional Centre, Islamabad, Pakistan, from April 2014 to April 2015. Methodology: Sixteen female rats (Sprague Dawley) were selected by random sampling method. Rats were mated and divided into control group A and experimental group B. From gestational day 10 to 18, mother rats received intraperitoneal injection of ethanol (Group B) and normal saline (Group A). Mother rats were allowed to complete their gestation and deliver spontaneously. When pups were born, only male pups were selected for the study. They were reared till day five. At 5ᵗʰ; day, pituitary glands were taken out and histological study was done in PAS-OG stain. Cell count was made in unit area (10,000 μ2) of pars distalis of pituitary gland. Student t-test was applied for analysing the data of cell counts in unit area. Results: Mean acidophil count was reduced in experimental group (70.19 ± 11.4) as compared to control group (92.65 ± 8.52, p<0.001). Mean basophil count was reduced in experimental group (27.05 ± 3.9) in comparison to control group (34.03 ± 7.9, p<0.001). Mean chromophobe count was increased (131.95 ± 10.7) in experimental group against the control group (104.62 ± 7.62, p < 0.001). Conclusion: Pups exposed to ethanol during gestation, showed significant reduction in acidophil and basophil count while increase in chromophobe cell count in pars distalis of pituitary gland.

research from:

• Foundation University Medical College

Year:

2019

Type of Publication:

Article

Journal:

Journal of the College of Physicians and Surgeons--Pakistan

Volume:

29

Number:

7

Pages:

600-603

Month:

7

DOI:

10.29271/jcpsp.2019.07.600