Background: Testosterone exerts a wide range of functions in males including behavioural traits like aggression, libido and sexual motivation. The objective of this study was to estimate serum Testosterone levels in Depressive male patients. Methods: This was a case control study performed at Islamic International Medical College, and Armed Forces Institute of Mental Health, Military Hospital Rawalpindi. The study comprised a total of 96 male participants having age of 18–60 years. They were divided into two groups: Group A consisted of 24 adult healthy males and Group B consisted of 72 male depressive patients diagnosed on the basis of Siddiqui Shah Depression Scale. Serum Testosterone of Group A was compared with that of Group B. Results: Serum Testosterone (7.98±1.03 ηg/ml) levels of Group A have shown no significant differences as compared to serum Testosterone levels (8.92±0.56 ηg/ml) of Group B. Conclusion: Serum testosterone levels were not found to be low in depressive patients.