Prevalence of carbapenemase-producing organisms at the Kidney Center of Rawalpindi (Pakistan) and evaluation of an advanced molecular microarray-based carbapenemase assay.

Shahid Ahmed Abbassi; Sascha D Braun; Bushra Jamil; Muhammad A Syed; Shahid A Abbasi; Daniel Weiß; Peter Slickers; Stefan Monecke; Ines Engelmann; Ralf Ehricht

Abstract:

Aim: A DNA microarray-based assay for the detection of antimicrobial resistance (AMR) genes was used to study carbapenemase-producing organisms at the Kidney Center of Rawalpindi, Pakistan. Methods: The evaluation of this assay was performed using 97 reference strains with confirmed AMR genes. Testing of 7857 clinical samples identified 425 Gram-negative bacteria out of which 82 appeared carbapenem resistant. These isolates were analyzed using VITEK-2 for phenotyping and the described AMR assay for genotyping. Results: The most prevalent carbapenemase gene was blaNDM and in 12 isolates we detected two carbapenemase genes (e.g., blaNDM/blaOXA-48). Conclusion: Our prevalence data from Pakistan show that – as in other parts of the world – carbapenemase-producing organisms with different underlying resistance mechanisms are emerging, and this warrants intensified and constant surveillance. Keywords: carbapenemasesCarbDetectCPOESBLhospitalmicroarrayPakistan

research from:

• Foundation University Medical College

Year:

2018

Type of Publication:

Article

Journal:

Future Microbiology

Volume:

13

Number:

11

Pages:

1

Month:

6